Browse Projects
Number of Projects: 3

26 Downloadable Samples



Bulk RNA-seq

DiagnosisEpendymoma (1), Ganglioglioma (5), Ganglioglioma/ATRT (1), Low-grade glioma (1), Pilocytic astrocytoma (18)

Pediatric brain tumors are now the most common cause of mortality from disease in childhood. Molecular characteristics of pediatric high- and low-grade gliomas (PHGG and PLGG), the most common tumor category overall, are crucial to treatment and outcomes, but the impact of these characteristics and of the variety of cell populations in these tumors is poorly understood. We performed single-cell RNA-sequencing on viably banked single cell samples of high- and low- grade glial tumors from children treated at Children’s Hospital Colorado. These samples are part of ongoing single-cell pediatric brain tumor banking that our group initiated a decade ago. The maturity of this resource, collected over a decade, provides us with the opportunity to perform well-powered outcome association studies. Samples are collected during routine surgery and immediately disaggregated to isolate single cells. These are then viably frozen in DMSO and banked for later use. We have tumors that cover the variety of subtypes in each of these diseases, as well as comprehensive clinical information on these cases, which will allow us to correlate molecular subtypes and research findings with these clinical measures. Here, we perform single-cell RNA-sequencing on 26 samples from patients with PLGG. In PLGG, we will leverage scRNA-Seq analysis to identify lineage specific development and subpopulations within these tumors. We will then evaluate single-cell RNA-sequencing signatures and clinical outcomes of LGG with BRAF WT, KIAA1549:BRAF fusion and BRAFV600E to identify unique drivers of aggressiveness of BRAFV600E tumors. These studies will significantly advance our understanding of disease biology and provide the detailed molecular and functional insights needed to identify new therapeutic targets for these biologically and clinically heterogeneous tumors.

PublicationsNot Specified
Also deposited underNot Specified
Additional Sample Metadata Fieldsdevelopment_stage_ontology_term_id, disease_ontology_term_id, Mutation_status, organism, organism_ontology_id, participant_id, self_reported_ethnicity_ontology_term_id, sex_ontology_term_id, spinal_leptomeningeal_mets, submitter, submitter_id, tissue_ontology_term_id

42 Downloadable Samples


10Xv2_5prime, 10Xv3.1

DiagnosisAtypical teratoid rhabdoid tumor (8), Focal cortical dysplasia (1), Ganglioglioma (8), Glial-neuronal tumor (2), High-grade glioma (1), Low-grade glioma (22)

Pediatric brain cancers are the most common solid tumors in children with varying survival rates depending on molecular features of cancer cells and associated microenvironment. Several studies have used single-cell RNA sequencing to define transcriptional programs active in cancer cells and highlighted supportive roles of non-cancerous cells embedded in these tumors. Although application of single-cell RNA sequencing technology has expanded our understanding of intratumoral heterogeneity and underlying transcriptional mechanisms in brain tumors at diagnosis, mechanisms of disease remains elusive. Therefore, the purpose of this proposal is to characterize cellular and transcriptional states of malignant and non-malignant compartments at primary diagnosis and at relapse. In this study, we performed single-nuclei sequencing of 42 patient samples taken at various disease stages (initial diagnosis, progressive disease, and relapsed disease) from 20 patients with low-grade glioma or Atypical Teratoid Rhabdoid Tumor (ATRT). Bulk whole genome and RNA-seq data for these samples are available from the Children’s Brain Tumor Network and the Gabriella Miller Kids First Data Resource Center. Findings from our study will help define the mechanistic shifts of tumor and non-cancerous cells over course of disease progression. Collectively with the single-cell Pediatric Cancer Atlas funded, this project lays the foundation of an important data set focused on lethal relapsed cancers that can continue to be expanded to identify cancer-intrinsic and extrinsic microenvironmental factors supporting recurrence of pediatric brain tumors.

PublicationsNot Specified
Also deposited underNot Specified
Additional Sample Metadata Fieldsage_at_last_known_clinical_status, chemotherapy, development_stage_ontology_term_id, disease_ontology_term_id, ethnicity, extent_tumor_resection, last_known_clinical_status, organism, organism_ontology_id, participant_id, race, radiation, sample_origin, self_reported_ethnicity_ontology_term_id, sex_ontology_term_id, submitter, submitter_id, surgery, tissue_ontology_term_id, treatment_details

38 Downloadable Samples



Bulk RNA-seq, Multiplexed

34 samples are multiplexed. Learn more

DiagnosisAnaplastic ependymoma (4), Anaplastic ganglioglioma (1), Desmoplastic ganglioglioma (2), Dysembryoplastic neuroepithelial tumor (3), Dysplastic gangliocytoma (1), Ependymoma (5), Ganglioglioma (1), Glioblastoma (1), Low-grade glioma (1), Medulloblastoma (4), Myxopapillary ependymoma (1), Non-cancerous (3), Pilocytic astrocytoma (6), Pilomyxoid astrocytoma (1), Primitive neuroectodermal tumor (3), Schwannoma (1), Subependymoma (1)

Single cell gene expression profiling of pediatric central nervous system (CNS) tumors holds great potential to further our understanding of carcinogenesis, augment prognostic indicators, and identify rational therapeutic targets. Whereas the genomic characteristics of these tumors are fairly well-defined in aggregate, the extent to which cellular heterogeneity is associated with carcinogenesis and clinical outcomes is largely unknown. Here we profile single nuclei gene expression in 36 brain tumor specimens from individuals with a diagnosis of ependymoma, glioma, or embryonal CNS tumor with substantial follow up time, as well as non-tumor brain tissue from three pediatric controls. We used the 10X Genomics Single Cell platform to obtain single nuclei for RNA sequencing in conjunction with bulk RNA sequencing. In conjunction with this study, we obtained 5-methyl- and 5-hydroxymethylation profiles on these samples to investigate functional aspects of gene regulation by cytosine modification. The data and results from this study are expected to reveal an abundance of information about pediatric CNS tumors with value for the broader scientific community.

Lee M. K., N. Azizgolshani, J. A. Shapiro, L. Nguyen, F. K. IV, et al., 2024 Identifying tumor type and cell type-specific gene expression alterations in pediatric central nervous system tumors. Nat Commun 15: 3634.
Lee M. K., N. Azizgolshani, Z. Zhang, L. Perreard, F. K. IV, et al., 2024 Associations in cell type-specific hydroxymethylation and transcriptional alterations of pediatric central nervous system tumors. Nat Commun 15: 3635.
Also deposited underSRP392501, GSE211362
Additional Sample Metadata FieldsDeveloped_recurrence, development_stage_ontology_term_id, disease_ontology_term_id, location_class, organism, organism_ontology_id, participant_id, self_reported_ethnicity_ontology_term_id, sex_ontology_term_id, submitter, submitter_id, tissue_ontology_term_id, WHO_grade, Years_to_recurrence
Alex’s Lemonade Stand Foundation for Childhood Cancer333 E. Lancaster Ave, #414, Wynnewood, PA 19096 USAPhone: 866.333.1213 • Fax: 610.649.3038Email: